Figure 8.

Effects of the ERK/CREB signaling pathways on A_2AR-induced expression of c-Ski in HMECs. Representative immunoblot images of the CGS21680-induced changes in the intracellular protein levels of PKA, p-PKA, ERK, p-ERK, CREB and p-CREB in HMECs after ERK/CREB inhibition. (B-E) Quantitative analysis of (A). **p < 0.01 (n=3); NS, not significant. (F) Immunohistochemistry for c-Ski (green) induced by treatment with CGS21680 in HMECs after ERK/CREB inhibition. Scale bar, 50 µm. (G) Representative images of tube formation 24 h after treatment with CGS21680. Scale bar, 50 µm. (H) Quantitative analysis of (G). **p < 0.01 (n=5); NS, not significant.