Figure 10.

Dietary restriction compromises zonation characteristics of periportal hepatocytes. (A) Subclusters of rat hepatocytes from Sham, Sham + DR and SG are illustrated in UMAPs. (B) Metabolism enrichment on each hepatocyte subcluster is displayed in a matrix. (C) Gene expression of Ctnnb1, Cnne1, Lrp5, Lrp6, Myc, Glul, Cyp2f2 and Cyp7a1 illustrated in rat hepatocytes. (D) Metabolism group-1 and -2 in rat hepatocytes. (E) Metabolism group-1 and -2 in the cell trajectory of rat hepatocytes. (F) Proportions of metabolism group-1 and -2 in rat hepatocytes of Sham, Sham + DR and SG groups. (G) Protein levels of Ki67, CYP2E1 and CYP7E1 illustrated in rat liver tissue (each view contains one or more portal and central areas) of Sham, Sham + DR and SG groups. (H) Relative differences of CYP2E1 and CYP7E1 levels between peri-central and -portal regions in each rat liver tissue of Sham, Sham + DR and SG groups. (I) Spatial distribution of fatty acid, bile acid and sphingolipid metabolites. In H&E- and IHC-characterized rat liver sample sections, periportal regions were outlined in red circles and pericentral regions were outlined in blue circles. Abbreviations: Sham: sham surgery; DR: dietary restriction; SG: sleeve gastrectomy; HepaC: hepatocyte; Met: metabolism; CYP: cytochrome. H&E: hematoxylin and eosin. '*' represents 'p < 0.05' and statistical significance.