Figure 7.

Sleeve gastrectomy facilitates lipid metabolism associated with myeloid cell infiltration in the liver. (A) The technical scheme of the deep-learning-based image analysis. The deep-learning approach assessed (B) proportions of hepatocytes, lymphocytes and myeloid cells in total liver cells per tissue section and (C) spatial distribution in representative liver samples. Regions with higher (red circles) and lower (green circles) myeloid cell densities were selected (D) Differentially generated metabolites were illustrated (higher myeloid cell infiltrated regions vs. lower myeloid cell infiltrated regions) in Sham, Sham + DR and SG groups. (E) Function enrichment of upregulated differentially generated metabolites (higher myeloid cell infiltrated regions vs. lower myeloid cell infiltrated regions) in the liver sample of SG group was analyzed based on the KEGG database. Abbreviations: C: control; Sham/S: sham surgery; DR: dietary restriction; SG: sleeve gastrectomy; KEGG: Kyoto encyclopedia of genes and genomes. The unpaired t-test, the Fisher's exact test and the one-way ANOVA test followed by Tukey's multiple comparison were performed. '*' represents 'p < 0.05' and statistical significance.