Figure 9.

Dietary restriction and sleeve gastrectomy alter the cellular differentiation of hepatocytes, cholangiocytes and hepatic stellate cells. (A) Cellular characteristic markers of HepaC (Pcna, Ppara, Cyp2e1, Cyp7a1 and Afp), Chol (Pcna, Sox9, Slc4a2, Hnf4a and Hnf1b) and HSC (Pdgfrb, Tgfb1, Col1a1, Acta2 and Vim) were displayed. (B) Cell trajectories of HepaC, Chol and HSC together with Cyp2e1 expression in HepaC, Pcna expression in Chol, and Pdgfrb expression in HSC. CK7- and IL-17A- expressing cells were (C) illustrated in situ and (D) quantified from total rat liver cells. Abbreviations: C: control; Sham/S: sham surgery; DR: dietary restriction; SG: sleeve gastrectomy; HepaC: hepatocyte; Chol: cholangiocyte; HSC: hepatic stellate cell; KEGG: Kyoto encyclopedia of genes and genomes. The one-way ANOVA test was performed. '*' represents 'p < 0.05' and statistical significance.