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. 2024 Aug 19;20(11):4566–4584. doi: 10.7150/ijbs.100905

Figure 2.

Figure 2

Knockdown of PUS1 Inhibits Invasion and Migration of Prostate Cancer Cells In Vitro. A Western blot analysis of PUS1 expression in the prostate epithelial cell line (RWPE-1) and prostate cancer cell lines, with GAPDH used as the loading control. B Western blot analysis showing PUS1 protein levels in DU145 and PC-3 cells following the treatments indicated. C&D Representative images (C) and bar graph analysis (D) of wound healing assays in DU145 and PC-3 cells following the indicated treatments. Scale bar, 20 μm. E-H Representative images (E&F) and bar graph analysis (G&H) of transwell migration/invasion assays in DU145 and PC-3 cells following the indicated treatments. I Representative immunofluorescence images of F-actin in DU145 and PC-3 cells following the indicated treatments. Scale bar, 5 μm. J&K Representative images (J) and bar graph analysis (K) of 3D tumor spheroid invasion assays in DU145 and PC-3 cells following the treatments indicated. Scale bar, 20 μm. All data are presented as the mean ± standard deviation (SD). *p<0.05, **p<0.01, ***p<0.001.