Figure 1.

SAS inhibits PD-L1 protein expression. Various murine or human malignant cells were cultured on FN-coated plates with or without SAS at various concentrations for 24 hours. The cells tested included (A) mouse B16/F10 melanoma cells, (B) human VLA-4-positive AML cells isolated from AML patients, (C) mouse D122 adenocarcinoma alveolar basal epithelial cells, and (D) Wehi-3B mouse myeloid monocytic leukemia cells. The cells were collected and stained with either PE-conjugated anti-mouse or anti-human PD-L1 antibodies or their respective isotype-matched controls. The results show one representative of 3 experiments. Each picture provided is accompanied by quantitative data from flow cytometry analysis. # p<0.001 vs. the isotype control; *p<0.05 vs. the PBS group. **p<0.01 vs. PBS.