Figure 4.

Inhibition of pAkt expression by SAS and its role in the inhibition of PD-L1 expression by the tellurium compound. (A) Mouse B16/F10 cells, either transfected with (B) control plasmid or (C) overexpressing (OE) Akt or (D, E) human VLA-4-positive AML cells isolated from AML patients or (F) AML cells overexpressing (OE) Akt or (G) transfected with control plasmid, were cultured on FN-coated plates with or without SAS or with the PI₃K inhibitor LY294002 (E) for 24 hours. The cells were collected, fixed, permeabilized and stained for pAkt (A, D) or PD-L1 (B, C, E-G). The results show one representative of 3 experiments. Akt (protein kinase B).