| Composition | Volume (500 mL total) | Final concentration | Comments |
|---|---|---|---|
| mTeSR 1 basal medium | 400 mL | – | – |
| mTeSR 1 5× supplement | 100 mL | 20% (vol/vol) | – |
| Dorso | – | 1 μM (1: 1,000) | Add just before use |
| SB-431542 | – | 10 μM (1: 1,000) | Add just before use |
| Y-27632 (ROCK inhibitor) | – | 5 μM (1: 1,000) | Add just before use. Only use on day 0 |
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