| Composition | Volume (525 mL total) | Final concentration | Comments |
|---|---|---|---|
| Neurobasal medium | 500 mL | – | M1 medium |
| GlutaMAX | 5 mL of 100× | 1× | |
| NEAA | 5 mL of 100× | 1% (vol/vol) | |
| Gem21 | 1 bottle (10 mL) | 2% (vol/vol) | |
| N2 | 1 bottle (5 mL) | 1% (vol/vol) | |
| Dorso | – | 1 μM (1: 1,000 of 1 mM stock) | Add just before use |
| SB-431542 | – | 10 μM (1: 1,000 of 10 mM stock) | Add just before use |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.