| Composition | Volume (520 mL total) | Final concentration | Comments |
|---|---|---|---|
| Neurobasal medium | 500 mL | – | M2 medium |
| GlutaMAX | 5 mL of 100× | 1× | |
| NEAA | 5 mL of 100× | 1% (vol/vol) | |
| Gem21 | 1 bottle (10 mL) | 2% (vol/vol) | |
| FGF | – | 20 ng/mL (1: 5,000 of 100 μg/mL stock) | Add just before use |
| EGF | – | 20 ng/mL (1: 5,000 of 100 μg/mL stock) | Add just before use |
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