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. Author manuscript; available in PMC: 2024 Sep 8.
Published in final edited form as: Nat Protoc. 2024 May 3;19(9):2712–2738. doi: 10.1038/s41596-024-00994-0
Composition Volume (510 mL total) Final concentration Comments
BrainPhys neuronal medium 500 mL
NeuroCult SM1 Neuronal Supplement 1 bottle (10 mL) 2% (vol/vol)