Figure 3.

Binding curves of hSP-A and rhSP-D to GAGs at different calcium concentrations. Top: binding curves of hSP-A in the presence of 0 mM (black), 2.5 mM (red), 5 mM (green), and 10 mM (blue) calcium to HA (A), HS (B), and CS (C). Bottom: binding curves of rhSP-D in the presence of 0 mM (black), 2.5 mM (red), 5 mM (green), and 10 mM (blue) calcium to HA (D), HS (E), and CS (F). Data are presented as means ± SEM of an n = 3–6 technical replicates. CS, chondroitin sulfate; GAG, glycosaminoglycan; HA, hyaluronan; HS, heparan sulfate; hSP-A, human SP-A.