Table 6.
Detection of the molecular assembly states of different surfactant proteins from iSCAM measurements
| MW from GE |
MW Form iSCAM |
||||
|---|---|---|---|---|---|
| Protein | Monomer | Species 1 | Species 2 | Species 3 | Species 4 |
| recSP-A1 | 54 kDa | 43% (63 kDa) | 14% (102 kDa) | 8% (141 kDa) | 5% (207 kDa) |
| recSP-A2 | 39 kDa | 22% (42 kDa) | 20% (85 kDa) | 13% (125 kDa) | 8% (197 kDa) |
| recSP-D | 43 kDa | 8% (54 kDa) | 31% (113 kDa) | 4% (234 kDa) | 33% (462 kDa) |
| hSP-A | 36 kDa | 27% (64 kDa) | 10% (147 kDa) | 30% (495 kDa) | 14% (1,009 kDa) |
| hSP-D | 43 kDa | 14% (57 kDa) | 23% (113 kDa) | 12% (254 kDa) | 28% (456 kDa) |
Percentage values represent counts within the Gaussian fit of each molecular weight population in proportion to the total counts of three measurements. GE, gel electrophoresis; iSCAM, interferometric light scattering microscopy; MW, molecular weight.