Figure 1.

Tissue dissection for molecular and imaging studies. Schematic of adipose tissue dissection for ex vivo live calcium imaging and immunofluorescence in the aortic perivascular adipose tissue (aPVAT; A), mesenteric perivascular adipose tissue (mPVAT; B), and white adipose tissue (WAT; C). Orange boxes are representative of dissected tissue taken for immunofluorescent staining. Magenta boxes are representative of tissue dissected for ex vivo live calcium imaging. Primary and secondary branching of the vasculature in the mPVAT is indicated by yellow arrows (B). Ao, aorta; A, artery; SI, small intestines; V, vein, 1st, primary branching; 2nd, secondary branching.