Figure 2.
Expression of neurotransmission-associated gene in adipose tissue. Heat map illustrating relative expression levels for synaptic transmission-related genes in 3 regions of mouse adipose tissue: aortic perivascular adipose tissue (aPVAT), mesenteric perivascular adipose tissue (mPVAT), and white adipose tissue (WAT). Expression levels represented are the averaged change in cycle threshold (ΔCt) for each gene of interest (n = 3 per sex/adipose region). Selected genes included synaptic ion channels, receptors, and transporters (Atp1b1, Cacn1c, Cacna2d1, Gria2, Grik3, Grik5, Hcn4, Kcnab2, Kcnip3, Slc6a17, Adra1a, Adrb1, Adrb2, Adrb3, Chrm1, Chrna7, Npy1r, Drd1, P2ry12), as well as proteins involved in synaptic vesicle formation (Nlgn1, Syn1) and synapse structure (Gphn, SHANK1, Dlg4). Expression levels in a single female cortex were included as confirmation of qPCR. Color scale indicates relative gene expression levels, ranging from highest (ΔCt = −4) to lowest (ΔCt = 18).