Figure 3.

Neural innervation in female and male mouse aortic perivascular adipose tissue (aPVAT). Immunofluorescent labeling of neuron fibers (anti-peripherin, magenta), vasculature (anti-CD31, yellow), and nuclei [4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), blue] in female and male aPVAT. A: representative confocal large area scan of stained female aPVAT (scale bar, 500 mm). B and C: ×10 images of labeled nerve fibers in female aPVAT (scale bar = 100 mm). D: representative large area scan of stained male aPVAT (scale bar = 500 mm). E: ×10 image of labeled nerve fibers in male aPVAT (scale bar = 100 mm). F and G: brightfield overlay of the large area scans for male (F) and female (G) mice. Ao, aorta. Representative of 3 females/males.