Figure 5.

Innervation of white adipose tissue (WAT). Immunofluorescent labeling of neuron fibers (peripherin, magenta), vasculature (CD31, yellow), and nuclei [4′,6-diamidino-2-phenylindole dihydrochloride (DAPI), blue] in female and male WAT. A: representative confocal large area scan of stained female WAT (scale bar = 500 mm). B–D: ×10 images of labeled nerve fibers in female WAT (scale bar = 100 mm). E: brightfield overlay of the large area scan for female WAT (A). F: representative large area scan of stained male WAT (scale bar = 500 mm). G and H: ×10 images of labeled nerve fibers in male WAT (scale bar = 100 mm). I: brightfield overlay of the large area scan for male mesenteric perivascular adipose tissue (mPVAT). Representative of 3 females/males.