Figure 6.

Visualization of innervation in cleared Adipoq^Cre−;GCaMP5g-tdT^fl/WT and noncleared NPY-GFP aortic perivascular adipose tissue (aPVAT). A: representative confocal large area scan of female Adipoq^Cre−;GCaMP5g-tdT^fl/WT aPVAT cleared and stained with anti-peripherin (magenta), anti-CD31 (yellow), lectin-649 (cyan), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue) (scale bar = 500 mm). B: ×10 image of a single frame within the large area scan (A) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). Representative confocal large area scan of aPVAT from female NPY-GFP mice expressing transgenically labeled NPY nerve fibers (yellow) and stained with lectin (cyan) and DAPI (blue, scale bar = 500 mm). D: ×10 image of a single frame within the NPY-GFP large area scan (C) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). Representative of 2 females for cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT and 1 female for NPY-GFP mice. Ao, aorta; LPVAT, lateral PVAT.