Figure 7.

Innervation in cleared Adipoq^Cre−;GCaMP5g-tdT^fl/WT and noncleared NPY-GFP mesenteric perivascular adipose tissue (mPVAT). A: representative large area scan of cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT mPVAT stained with anti-peripherin (magenta), anti-CD31 (yellow), lectin-649 (cyan), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue) (scale bar = 500 mm). B and C: ×10 images of a single frame within the cleared mPVAT large area scan (A) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). D: representative large area scan of noncleared mPVAT from female NPY-GFP mice expressing transgenic labeling of NPY nerve fibers (yellow) and stained with lectin (cyan) and DAPI (blue, scale bar = 500 mm). E and F: ×10 images of a single frame within the NPY-GFP large area scan (D) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). Representative of 2 females for cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT and 1 female for NPY-GFP.