Figure 8.

Neural innervation in cleared Adipoq^Cre−;GCaMP5g-tdT^fl/WT and noncleared NPY-GFP white adipose tissue (WAT). A: representative large area scan of cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT WAT stained with anti-peripherin (magenta), anti-CD31 (yellow), lectin (cyan), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue) (scale bar = 500 mm). B: ×10 image of a single frame within the cleared WAT large area scan (A) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). Representative large area scan of noncleared WAT from female NPY-GFP mice expressing transgenic labeling of NPY nerve fibers (yellow) and stained with lectin (cyan) and DAPI (blue, scale bar = 500 mm). D: ×10 image of a single frame within the NPY-GFP large area scan (C) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). Representative of 2 females for cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT and 1 female for NPY-GFP.