Figure 9.

Neural innervation in mesenteric perivascular adipose tissue (mPVAT) in additional transgenic mouse models. Transgenic expression of fluorescent proteins in nerves present in the mPVAT in Wnt1Cre⁺:GCaMPtdT^f/Wt female mice. Traditional immunofluorescence with 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI) counterstaining and without vascular staining was used. A: representative confocal large area scan of mPVAT from Wnt1Cre⁺:GCaMPtdT^f/Wt female mice expressing tdTomato and GCaMP5g expression in nerve fibers, counterstained with DAPI (scale bar = 500 mm, n = 3 females). B and C: ×10 images of single frames within the Wnt1Cre⁺:GCaMPtdT^f/Wt large area scan depicting labeled nerve fibers and nuclei (scale bar = 100 mm). D: brightfield overlay of mPVAT obtained from transgenic Wnt1Cre⁺:GCaMPtdT^f/Wt female mice expressing tdTomato and GCaMP5g in nerve fibers and counterstained with DAPI (A). A, artery; SI, small intestines; V, vein. Representative of 3 females.