Figure 10.

Sympathetic innervation in cleared Adipoq^Cre−;GCaMP5g-tdT^fl/WT mesenteric perivascular adipose tissue (mPVAT) and white adipose tissue (WAT). TH, tyrosine hydroxylase. A: representative confocal large area scan of cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT mPVAT stained with anti-TH (magenta), lectin (cyan), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue) (scale bar = 500 mm). B and C: ×10 image of a single frame within the cleared mPVAT large area scan (A) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). D: representative confocal large area scan of cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT WAT stained with anti-TH (magenta), lectin (cyan), and DAPI (blue) (scale bar = 500 mm). E: ×10 image of a single frame within the cleared WAT large area scan (D) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). F: ×20 image of a single frame within the cleared WAT large area scan (D) depicting labeled nerve fibers and vasculature (scale bar = 50 mm). Representative of 2 females.