Figure 11.

Sympathetic innervation in cleared Adipoq^Cre−;GCaMP5g-tdT^fl/WT aortic perivascular adipose tissue (aPVAT). TH, tyrosine hydroxylase. A: representative confocal large area scan of cleared female Adipoq^Cre−;GCaMP5g-tdT^fl/WT aPVAT stained with anti-TH (magenta), lectin (cyan), and 4′,6-diamidino-2-phenylindole dihydrochloride (DAPI, blue) (scale bar = 1,000 mm). B and C: ×10 image of a single frame within the cleared aPVAT large area scan (A) depicting labeled nerve fibers and vasculature (scale bar = 100 mm). D and E: ×40 image of a single frame within the cleared aPVAT large area scan (A) depicting labeled nerve fibers and vasculature (scale bar = 25 mm). Ao, aorta; LP, lateral PVAT. Representative of 2 females.