Figure 13.
Transgenic expression of reporter protein, tdTomato (tdT), and genetically encoded calcium indicator, GCaMP5g, in adipocytes of AdipoqCre+;GCaMPtdTf/Wt transgenic mice. A and B: representative images of aortic perivascular adipose tissue (aPVAT), mesenteric perivascular adipose tissue (mPVAT), and white adipose tissue (WAT) from male (A) and female (B) AdipoqCre+;GCaMPtdTf/Wt mice demonstrating the expression of the reporter protein tdTomato and the genetically encoded calcium indicator, GCaMP5g, in mesenteric adipocytes (scale bar = 100 µm). Representative images of calcium responses in male (A) and female (B) mice to stimulation with 10 µM bethanechol, electrical field stimulation (EFS, +100 V, 0.1 ms, 10 Hz, 1 pulse), and 10 µM norephinephrine (NE).