Table 1.

Expression of genes associated with neurotransmission in adipose tissue

	aPVAT		mPVAT		WAT		Cortex	Region		Sex
Gene ID	ΔCt female	ΔCt male	ΔCt female	ΔCt male	ΔCt female	ΔCt male	ΔCt female	F	P value	F	P value
Ion channels, receptors, transporters
Atplbl	3.574 ± 0.227	4.579 ± 0.126	3.555 ± 1.140	4.283 ± 0.768	5.042 ± 0.352	4.945 ± 0.221	−2.2715	0.924	0.0528	2.513	0.1389
Cacnlc	8.38 ± 0.355c,d	8.987 ± 0.258a,b	7.386 ± 0.615	7.538 ± 0.190	7.356 ± 0.071	7.221 ± 0.185	3.1955	21.44	0.0001	1.203	0.2943
Cacna2dl	7.024 ± 0.676	7.963 ± 0.289	6.411 ± 0.563	6.475 ± 0.281	6.764 ± 0.135	6.635 ± 0.106	1.271	7.455	0.0079	1.58	0.2327
Gria2	12.999 ± 0.661	15.518 ± 1.165e	13.945 ± 0.317	12.910 ± 0.706f,g	16.645 ± 0.63 lh,i	15.505 ± 0.284	−0.204	15.32	0.0005	0.0818	0.7798
Grik3	12.181 ± 0.716	12.685 ± 0.531	11.617 ± 0.708	10.986 ± 0.385j,k	13.002 ± 0.279	13.277 ± 0.510	3.961	11.58	0.0016	0.0245	0.8781
Grik5	7.126 ± 0.850	7.572 ± 0.331	6.859 ± 0.597	6.983 ± 0.201	7.294 ± 0.128	7.466 ± 0.213	2.09	1.22	0.3292	0.8525	0.374
Hcn4	11.649 ± 0.171	12.496 ± 0.221l,m	11.332 ± 1.000	11.032 ± 0.402	9.637 ± 0.466n,o	10.128 ± 0.516	8.299	16.9	0.0003	1.25	0.2854
Kcnab2	13.038 ± 0.783p,q	13.594 ± 0.439r,s	9.040 ± 0.637	9.848 ± 0.085t	10.369 ± 0.436	11.733 ± 0.380u	3.059	58.46	<0.0001	9.581	0.0093
Kcnip3	8.401 ± 0.719	7.401 ± 0.246v	7.713 ± 0.662	8.110 ± 0.354	8.118 ± 0.198	7.800 ± 0.150	1.167	0.019	0.9811	1.41	0.2581
Slc6a17	11.631 ± 0.689	11.730 ± 0.297w	9.995 ± 0.740	9.470 ± 0.244	10.365 ± 0.336	10.440 ± 1.632	1.38	5.96	0.0159	0.0625	0.8068
Adrala	3.268 ± l.383x,y	3.585 ± 0.294z,A	7.070 ± 1.040	7.332 ± 0.399	5.699 ± 0.344	6.835 ± 0.442	4.345	26.1	<0.0001	1.654	0.2227
Adrbl	4.144 ± 1.639B	4.421 ± 0.497	6.364 ± 0.613	4.540 ± 0.574	6.686 ± 0.791	5.248 ± 0.875	2.9	3.558	0.0612	3.544	0.0842
Adrb2	4.389 ± 0.178	3.768 ± 0.391	4.767 ± 0.096	4.273 ± 0.767	4.934 ± 0.197	4.479 ± 0.217	5.748	2.645	0.1118	5.685	0.345
Adrb3	5.333 ± 1.027	5.526 ± 0.627C	4.609 ± 0.636	4.314 ± 0.185	3.960 ± 0.281	3.189 ± 0.251	12.327	10.19	0.0026	0.751	0.4031
Chrml	11.346 ± 0.674	12.201 ± 1.204	10.309 ± 0.242	10.747 ± 0.530	11.570 ± 0.155	10.693 ± 0.274	0.915	3.977	0.0473	0.148	0.7072
Chrna7	11.656 ± 0.295	12.244 ± 0.838	12.472 ± 0.529	11.878 ± 0.303	12.166 ± 0.395	13.143 ± 0.389	3.448	3.698	0.0561	1.287	0.2787
Npylr	11.497 ± 0.214D,E	11.352 ± 0.404F	9.938 ± 0.902	9.276 ± 0.134	10.018 ± 0.219	10.354 ± 0.304	6.358	17.51	0.0003	0.3753	0.5516
Drdl	11.862 ± 0.889G,H	12.494 ± 0.606I,J	9.426 ± 0.457	8.636 ± 0.324	9.726 ± 0.587	7.021 ± 0.381K,L	2.72	50.57	<0.0001	8.35	0.0136
P2ry12	14.073 ± 0.303M,N	13.615 ± 0.244	13.239 ± 0.343	13.134 ± 0.229	13.270 ± 0.176	13.127 ± 0.348	9.319	7.162	0.009	2.107	0.1723
Synaptic vesicles
Nlgnl	9.298 ± 0.157O,P	9.112 ± 0.140Q,R	8.137 ± 0.397	7.526 ± 0.110S	7.799 ± 0.165	6.471 ± 0.264T,U	1.683	85.63	<0.0001	29.02	0.0002
Syn	6.336 ± 0.955	6.451 ± 0.358	7.138 ± 0.622	6.702 ± 0.143	6.538 ± 0.130	6.969 ± 0.386	−1.061	1.078	0.3711	0.0147	0.9054
Synapse structure
Gphn	4.779 ± 0.526	5.487 ± 0.397	4.990 ± 0.527	4.646 ± 0.155	5.141 ± 0.132	4.534 ± 0.154	2.935	0.9632	0.4093	0.1511	0.7043
SHANK1	10.668 ± 0.046V	10.046 ± 0.265	9.165 ± 0.622	9.295 ± 0.207	9.376 ± 0.526	10.155 ± 0.886	0.031	4.878	0.0282	0.1058	0.7506
Dlg4	7.856 ± 0.558	7.724 ± 0.192	6.670 ± 0.523	6.424 ± 0.102	6.732 ± 0.116	6.604 ± 0.170	−0.376	16.72	0.0003	0.7573	0.4012

Values are means ± SD, with the degrees of freedom (F) and corresponding P value for two factors included in the statistical analyses: region and sex; n = 3 experimental males/females per region of adipose tissue, and n = 1 female for cortical tissue quantification of change in cycle threshold (ΔCt) values for all genes examined in male and female aortic mesenteric perivascular adipose tissue (aPVAT), aortic and mesenteric PVAT (mPVAT), white adipose tissue (WAT), and female cortical tissue. No statistical measures were performed using the cortical data generated from a signal female (n = 1). Statistical analysis comparing region and sex for each individual gene, not between genes, was performed using two-way ANOVA followed by Tukey’s multiple comparison test.

^aP = 0.0023 vs. male mPVAT,

^bP = 0.0005 vs. male WAT,

^cP = 0.0224 vs. female mPVAT,

^dP = 0.0264 vs. female WAT,

^eP = 0.0034 vs. female aPVAT,

^fP = 0.0070 vs. male aPVAT,

^gP = 0.0072 vs. male WAT,

^hP = 0.0005 vs. female aPVAT,

ⁱP = 0.0055 vs. female WAT,

^jP = 0.0225 vs. male aPVAT,

^kP = 0.0032 vs. male WAT,

^lP = 0.0447 vs. male mPVAT,

^mP = 0.0022 vs. male WAT,

ⁿP = 0.0072 vs. female aPVAT,

^oP = 0.0206 vs. female mPVAT,

^pP < 0.0001 vs. female mPVAT,

^qP = 0.0006 vs. female WAT,

^rP < 0.0001 vs. male mPVAT,

^sP = 0.0085 vs. male WAT,

^tP = 0.0079 vs. male mPVAT,

^uP = 0.0200 vs. female WAT,

^vP = 0.0453 vs. female aPVAT,

^wP = 0.0405 vs. male mPVAT,

^xP = 0.0009 vs. female mPVAT,

^yP = 0.0208 vs. female WAT,

^zP = 0.0010 vs. male mPVAT,

^AP = 0.0031 vs. male WAT,

^BP = 0.0412 vs. female WAT,

^CP = 0.0045 vs. male WAT,

^DP = 0.0111 vs. female mPVAT,

^EP = 0.0153 vs. female WAT,

^FP = 0.0014 vs. male mPVAT,

^GP = 0.0029 vs. female mPVAT,

^HP = 0.0074 vs. female WAT,

^IP < 0.0001 vs. male mPVAT,

^JP < 0.0001 vs. male WAT,

^KP = 0.0379 vs. male mPVAT,

^LP = 0.0005 vs. female WAT,

^MP = 0.0294 vs. female mPVAT,

^NP = 0.0359 vs. female WAT,

^OP = 0.0007 vs. female mPVAT,

^PP < 0.0001 vs. female WAT,

^QP < 0.0001 vs. male mPVAT,

^RP < 0.0001 vs. male WAT,

^SP = 0.0199 vs. female mPVAT,

^TP = 0.0015 vs. male mPVAT,

^UP < 0.0001 vs. female WAT, and

^VP = 0.0306 vs. female mPVAT.