Figure 8.

Site-directed mutagenesis identified CD8α epitopes recognized by anti-CD8 mAbs. CDα mutations that influence mAB binding are shown as light blue spheres. From top left to bottom right: mouse CDα mutations at residues R8, K21, D23, and T81 (ectodomain numbering) reduce binding to 53.6.7 antibody, mouse CDα mutations at residues R8, K12, and K13 (ectodomain numbering) reduce binding to H59.101 antibody, and mouse CDα mutations at residues K62 (ectodomain numbering) reduce binding to KT15 and CT-CD8a antibodies. As a reference, mutations are plotted onto CDα from mouse CD8αβ/H-2D^d (PDB ID 3DMM). MHC-I heavy chains are colored green, β2m light chains are colored cyan, peptide antigens are colored salmon, CD8α chains are colored magenta, CD8β chains are colored yellow, and the mABs names are colored blue. Data were derived from Devine et al. (52).