Figure 8.

The roles of rid1 and sae2 in initiation and repair of DSB1. (A) Restriction map of DSB1 in the first chromosome of QM6a and CBS999.97(MAT1-1). Polymorphic DNA sequences in QM6a and CBS999.97(MAT11-1) are indicated in red and blue, respectively. The BglII restriction enzyme sites and the two break sites revealed by our BND ssDNA enrichment experiment are indicated by green arrowheads and ‘X’, respectively. After BglII digestion, the expected fragment sizes for the full-length bands, unprocessed DSB, and ssDSB are designated. The location of the DNA probe (red bar) used for Southern hybridization is shown above the chromosomes. (B, C) Southern hybridization of gDNA isolated from two haploid maternal and paternal vegetative mycelia (VM) and the corresponding fruiting bodies (FBs) at indicated days after the initiation of sexual crosses, as well as the mature ascospores released from wild-type and sae2Δ FBs. Visualization/quantification of Southern hybridization band intensity was performed using a BAS-IP MS204 phosphorimaging plate (Cytiva, Japan) and a TyphoonFLA 9000 biomolecular imager (Cytiva, Japan).