Figure 9.

The roles of rid1 and spo11 in initiation and repair of DSB1 (Figure 8A). (A) The smp3 DNA was used as the loading control for Southern hybridization. (B) Southern hybridization of gDNA isolated from eight haploid maternal and paternal vegetative mycelia (VM). (C) Southern hybridization of gDNA isolated from the corresponding fruiting bodies (FBs) at indicated days after the initiation of sexual crosses, as well as the mature ascospores released from the indicated fruiting bodies. Visualization/quantification of Southern hybridization band intensity was performed using a BAS-IP MS204 phosphorimaging plate (Cytiva, Japan) with a Typhoon 5 biomolecular imager (Cytiva, Japan).