Table 1.

Summary of the phenotypes of different sexual crosses

Sexual crosses
MAT1-1				MAT1-2
WTH strain number and genetic background		Genotype	ham5 a	WTH strain number and genetic background		Genotype	ham5 a	Fruiting bodies	Mature asciib	Mature sporesc	No. of dissected ascii/spores	No. of viable sexual spores	Sexual pore viability (%)
A
0011	C(re)d	wild type (wt)	+	0015	Qd	wt	−	+	+++	+++	30/480	356	74
0011	C (re)	wt	+	0013	C	wt	+	+	+++	+++	30/480	332	69
0011	C (re)	wt	+	0282	C (re)	wt	+	+	+++	+++	20/320	320	100
0270	C	wt	+	0013	C	wt	+	+	+++	+++	20/320	320	100
7205	H	spo11Δ	+	7217	H (re)	spo11Δ	+	+	+++	+++	39/624	480	77
7205	H	spo11Δ	+	10586	H	spo11Δ	–	+	+++	+++	26/416	416	100
10610	H (re)	spo11Δ	+	10586	H	spo11Δ	–	+	+++	+++	5/80	57	71
12794	H	rad51Δ	−	12867	H (re)	rad51Δ	+	+	+++	−	−	−	−
0011	C (re)	wt	+	12867	H (re)	rad51Δ	+	+	+++	+++	5/80	56	70
12902	H (re)	spo11Δrad51Δ	−	12901	H (re)	spo11Δrad51Δ	+	+	+++	−	−	−	−
13049	H	sae2Δ	+	13040	Q	sae2Δ	+	+	+++	+	26/232	23	10
13049	H	sae2Δ	+	12867	H (re)	rad51Δ	+	+	+++	+++	3/48	19	40
13053	H	spo11Δsae2Δ	−	13054	H	spo11Δsae2Δ	+	+	+++	+	26/230	34	15
B
0011	C (re)	wt	+	13058	Q	rid1Δ	−	+	+++	+++	3/48	34	71
0011	C (re)	wt	+	13060	Q	dim2Δ	−	+	+++	+++	5/80	60	80
0011	C (re)	wt	+	13215	Hd	dim2 rid1Δ	+	+	+++	+++	5/80	60	80
13059	C (re)	rid1Δ	+	0015	Q	wt	−	+	+++	+++	2/32	24	75
13059	C (re)	rid1Δ	+	13058	Q	rid1Δ	−	+	+	−	−	−	−
13059	C (re)	rid1Δ	+	13060	Q	dim2Δ	−	+	+++	+++	4/64	52	81
13059	C (re)	rid1Δ	+	13215	H	dim2Δrid1Δ	+	+	+	−	−	−	−
13079	H	dim2Δ	+	0015	Q	wt	−	+	+++	+++	5/80	76	95
13079	H	dim2Δ	+	13058	Q	rid1Δ	−	+	+++	+++	5/80	63	79
13079	H	dim2Δ	+	13060	Q	dim2Δ	−	+	+++	+++	5/80	73	91
13079	H	dim2Δ	+	13215	H	dim2Δrid1Δ	+	+	+++	+++	3/48	48	100
13147	H (re)	dim2Δrid1Δ	+	0015	Q	wt	−	+	+++	+++	2/32	23	72
13147	H (re)	dim2Δrid1Δ	+	13058	Q	rid1Δ	−	+	+	−	−	−	−
13147	H (re)	dim2Δrid1Δ	+	13060	Q	dim2Δ	−	+	+++	+++	10/160	123	77
13147	H (re)	dim2Δrid1Δ	+	13215	H	dim2Δrid1Δ	+	+	−	−	−	−	−
13059	C (re)	rid1Δ	+	10586	H	spo11Δ	−	+	+++	+++	10/160	104	65
13079	H	dim2Δ	+	10586	H	spo11Δ	−	+	+++	+++	4/64	46	72
13289	H	spo11Δrid1Δ	+	13290	H	spo11Δrid1Δ	+	+	+++	−	−	−	−
13269	H (re)	spo11Δdim2Δ	+	13268	H (re)	spo11Δdim2Δ	−	+	+++	+++	3/48	43	90
C
13059	C (re)	rid1Δ	+	12867	H (re)	rad51Δ	+	+	+++	+/−	−	−	−
13059	C (re)	rid1Δ	+	12901	H (re)	spo11Δrad51Δ	+	+	+++	−	−	−	−
12794	H	rad51Δ	−	13290	H	spo11Δrid1Δ	+	+	+	−	−	−	−
13297	H	rad51Δ	+	13058	Q	rid1Δ	−	+	+++	+/−	−	−	−
13289	H	spo11Δrid1Δ	+	12867	H (re)	rad51Δ	+	+	+++	+/−	−	−	−
13289	H	spo11Δrid1Δ	+	12901	H (re)	spo11Δrad51Δ	+	+	+++	+	−	−	−
13299	H	rad51Δrid1Δ	+	13315	H	rad51Δrid1Δ	+	+	+++	−	−	−	−
13287	H	rad51Δdim2Δ	+	13286	H	rad51Δdim2Δ	+	+	+++	−	−	−	−
13059	C (re)	rid1Δ	+	13040	H	sae2Δ	+	+	+++	+	7/58	38	66
13079	H	dim2Δ	+	12867	H (re)	rad51Δ	+	+	+++	+++	4/64	42	66
13269	H (re)	spo11Δdim2Δ	+	12867	H (re)	rad51Δ	+	+	+++	+++	4/64	52	81
13147	H (re)	dim2Δrid1Δ	+	12867	H (re)	rad51Δ	+	+	+++	+++	6/96	92	96
13059	C (re)	rid1Δ	+	13286	H	rad51Δdim2Δ	+	+	+++	+/-	−	−	−
12794	H	rad51Δ	−	13215	H	rid1Δdim2Δ	+	+	+++	+++	3/48	45	94
13287	H	rad51Δ dim2Δ	+	13058	Q	rid1Δ	+	+	+++	+/-	3/48	46	96
13287	H	rad51Δdim2Δ	+	13215	H	rid1Δdim2Δ	+	+	+++	+++	4/64	64	100
13147	H (re)	dim2Δrid1Δ	+	13286	H	rad51Δdim2Δ	+	+	+++	+/-	3/47	14	30
D
0270	C	wt	+	13352	Q	msh4Δ	-	+	+++	+++	11/176	101	68
13297	H	rad51Δ	+	13352	Q	msh4Δ	-	+	+++	+++	8/128	82	64
13359	H	msh4Δ	-	12867	C (re)	rad51Δ	+	+	+++	-	-	-	-
13359	H	msh4Δ	-	13354	H	msh4Δ	+	+	+++	+++	8/128	97	76

^a ham5+ encodes a wild-type HAM5 protein, whereas ham5− encodes a truncated protein of only 151 amino acids due to a point mutation (G₅₃₁ → T) at the first position of the second intron, thus changing the invariant G₊₁ of the donor splicing site (53).

^b+++: the majority of asci were >50 μm in length; +: <10% asci were >50 μm; −: no elongated asci.

^c+++: the majority of asci contain 16 mature ascospores; +: the majority of asci (e.g. sae2Δ/sae2Δ or spo11Δsae2Δ/spo11Δsae2Δ) formed <16 mature ascospores; +/−: very few (<1%) asci formed mature ascospores; −: the elongated asci hardly formed any mature ascospores.

^dQ represents QM6a (MAT1-2) and the gene deletion mutants that are directly generated from this wild isolate by homologous recombination.

^eC represents CBS999.97(MAT1-1, re), CBS999.97(MAT1-1, wt), and the gene deletion mutants directly generated from these two wild isolates by homologous recombination, with ‘re’ and ‘wt’ representing reciprocal exchange and wild type, respectively (11,56).

^fQM6a (MAT1-2, wt), CBS999.97(MAT1-2, wt), and their mutants are denoted by ‘wt’, whereas T. reesei mutants carrying the same reciprocal exchange as the CBS999.97(MAT1-1, re) wild isolate are denoted by ‘re’ (11,56).

^gH represents the hybrid haploid mutants generated from sexually crossing wild-type CBS999.97(MAT1-1, re) with the QM6a (MAT1-2, wt) mutant or wild-type QM6a (MAT1-2, wt) with the CBS999.97(MAT1-1, re) mutant (11,56).