Figure 4.

SUMOylation inhibition modifies AR occupancy on chromatin. (A) Heatmap of AR’s normalized tag densities on moderately SUMOi-affected ARBs (C4, – 3 < log₂[SUMOi/CTRL] < 3), clearly SUMOi-attenuated ARBs (C5, log₂[SUMOi/CTRL] < −3) and SUMOi-enhanced ARBs (C6, 3 < log₂[SUMOi/CTRL]) together with SUMO2/3 occupancy, chromatin accessibility and H3K27ac data. (B–E) Box plots of AR, SUMO2/3, ATAC and H3K27ac tag densities in clusters C4–C6. (F) Chromatin occupancy of FOXA1 and HOXB13 in the presence and absence of DHT and SUMOi in clusters C4–C6 as box plots of tag densities. Statistical significance for tag density box plots calculated with one-way ANOVA with Bonferroni post hoc test and shown as asterisks with *P< 0.05, **P< 0.01 and ***P< 0.001.