Figure 4.
Radial distribution function g(r) analysis of Pol Y1-Halo and DnaX-mYPet colocalization. (A) Pol Y1-DnaX g(r) in untreated cells for all Pol Y1 trajectories, static Pol Y1 trajectories (D* < 0.14 μm2/s), and mobile Pol Y1 trajectories (D* > 0.14 μm2/s). (B) Pol Y1-DnaX g(r) for WT Pol Y1, Pol Y1-CBM-mut1, Pol Y2-CBM-mut2 and Pol Y1-cat-mut in untreated cells. (C) Pol Y1-DnaX g(r) in untreated cells and cells treated with 1 μM 4-NQO for 1 h, 10 μM 4-NQO for 1 h, or 10 μM 4-NQO for 2 h. Random g(r) curves are shown as dashed lines. As described in the Methods section, g(r) > 1 indicates colocalization, whereas g(r) = 1 indicates no colocalization.