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. 2024 Jul 25;52(16):9613–9629. doi: 10.1093/nar/gkae637

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© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — Apparent diffusion coefficient (D*) distributions for Pol Y1-Halo, determined from trajectory MSD values as described in the Methods section, and corresponding three-population fits. D* distributions for WT Pol Y1 in untreated cells for (A) all molecules, (B) molecules < 200 nm from DnaX-mYPet foci and (C) molecules > 200 nm from DnaX-mYPet foci. (D) D* distributions for WT Pol Y1 in cells treated with 10 μM 4-NQO for 1 h. D* distributions for (E) Pol Y1-CBM-mut1, (F) Pol Y1-CBM-mut2, and (G) Pol Y1-cat-mut mutants in untreated cells. (H) Difference between D* distributions for WT Pol Y1 in 4-NQO-treated and untreated cells. Note different y-axis scales in panels (B) and (H).