Table I.

STAT3 primers for PCR, Sanger sequencing, and site-directed mutagenesis

PCR for full-length STAT3 cDNA
Name	Sequences (5'–3')
STAT3 forward	GCCGCCGCCATGGCCCAATGGAATCAGC
STAT3 reverse	CATGGGGGAGGTAGCGCACTCCGAGGTC
Sanger sequencing
Name	Sequences (5'–3')
T7	TAATACGACTCACTATAGGG
STAT3 seq 1 forward	AAACTGGATAACGTCATTAGCAGAATCTCA
STAT3 seq 1 reverse	TGAGATTCTGCTAATGACGTTATCCAGTTT
STAT3 seq 2 forward	CTGGCAGAGAAACTCTTGGGACCTGGTGTG
STAT3 seq 2 reverse	CACACCAGGTCCCAAGAGTTTCTCTGCCAG
BGH reverse	TAGAAGGCACAGTCGAGG
Site-directed mutagenesis
Name	Sequences (5'–3')
c.2144C＞T (p.P715L) forward	GTGACACTAACGACCTGCAGCAATAC
c.2144C＞T (p.P715L) reverse	GGTCGTTAGTGTCACACAGATAAACT
c.2144C＞G (p.P715R) forward	GTGACACGAACGACCTGCAGCAATAC
c.2144C＞G (p.P715R) reverse	GGTCGTTCGTGTCACACAGATAAACT
c.2143C＞T (p.P715S) forward	TGTGACATCAACGACCTGCAGCAATA
c.2143C＞T (p.P715S) reverse	GTCGTTGATGTCACACAGATAAACTT
c.2143C＞A (p.P715T) forward	TGTGACAACAACGACCTGCAGCAATA
c.2143C＞A (p.P715T) reverse	GTCGTTGTTGTCACACAGATAAACTT
c.2143C＞G (p.P715A) forward	TGTGACAGCAACGACCTGCAGCAATA
c.2143C＞G (p.P715A) reverse	GTCGTTGCTGTCACACAGATAAACTT
c.2147C＞T (p.T716M) forward	ACACCAATGACCTGCAGCAATACCAT
c.2147C＞T (p.T716M) reverse	GCAGGTCATTGGTGTCACACAGATAA
c.1909G＞A (p.V637M) forward	CCAGTCCATGGAACCATACACAAAGC
c.1909G＞A (p.V637M) reverse	GGTTCCATGGACTGGATCTGGGTCTT