Fig. 4.

Fibrosis in l-NAME-induced RV hypertrophy. A) Sirius red staining visualizing the amount of collagen expression in both ventricle. Scale bars correspond to 100 μm. B and C) Quantification of Sirius Red Staining as arbitrary units (AU). One-Way ANOVA (p < 0.000) and Student-Newman-Keuls post hoc analysis; #, p < 0.05 vs. L, LS, C; *, p < 0.05 vs. C (n = 24 slices from four independent animals per group). D-E) COL1A1 expression normalized to control values; LV: Kruskall-Wallis (p = 0.001); *, p < 0.05 vs. C with Bonferroni-Test with correction for multiple testing (LS vs. C: p = 0.000251); RV: Kruskal-Wallis-Test p = 0.033; *, p < 0.05 vs. C with Mann-Whitney-U-Test (LS vs. C: p = 0.010; C, n = 11, l-NAME, n = 13; LS and LT, n = 8 each). Data show means ± SD in black and original data points in red. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)