Fig. 4.

LiECs support formation and growth of hepatobiliary organoids by paracrine manner. (A) Schematic depicting organoid culture using Transwell from 5000 single cells of HO at P0. (B) Representative stitched image of HO and HO:LiEC at day 7 (passage 1). Scale bar, 1 mm. (C) Quantitation of the total number of whole organoids formed in HO and HO:LiEC, categorized by size (100–200 μm, >200 μm, and total) and (D) the average organoid size at day 7 (passage 1). Data are means ± SEM obtained from n = 3 independent biological replicates. *p < 0.05; **p < 0.01.