Figure 2.

The FUCCI system. (A) The transgene contains the Cdt1-linked mKO2 and the Geminin-linked mAG markers. (B) This way the cells express red fluorescence in the G1 phase, changes to orange in S and to green in G2. At the beginning of mitosis (M) the fluorescence fades and the cell divides. (C) After the electroporation, the fluorescent cell ratio was 26% in the male, and 32% in the female line, but this was not equal to the transgenic ratio. Since during the M phase, the cells lose their fluorescence, part of the non-fluorescent cell mass is a group of transgenic cells under mitosis. (D) In the clone cell lines, the ratio changed compared to the original transgenic lines. The male line contained twice as many fluorescent cells compared to the whole cell number, than the female line. (E,F) With the TO-PRO-3 nuclear staining, the non-fluorescent cells were also visualized to show the presence of cells in different cell cycle stages simultaneously in the culture.