Figure 3.

Characterization of the clone cell lines. (A) Germ- and stem cell specific gene expression in the original transgenic and clone cell lines. Both the original transgenic 1116 and 1111 lines expressed the CVH, DAZL and POUV markers. (B) The chromosome staining showed no degradation of the sexual chromosomes due to the long term culture in the clone cell lines. (C,D) Their integration into recipient gonads was successful, therefore transgenic gonadal chimaera embryos could be produced using the clone lines. (E) Two copies of the FUCCI construct were found in the FCM5 male line, both of them in an intron region. The insertion on chromosome 12 was a full insertion, while the one on chromosome 2 cannot be proven as a whole integration. (F) In case of FCF5, only one integration was found in an exon region.