Figure 5.

Sex-specific dependencies on the MPC for adipose glycerol-3-phosphate synthesis in vivo. (A - D) In vivo incorporation of intraperitoneally administered [U–¹⁴C] glucose into the glycerol moieties of total lipids (glyceroneogenesis A, B, D) or polar metabolites (C) in inguinal adipose (A), epididymal adipose (B - C), or liver (D) for male and female Mpc1^AD−/− mice or LoxP^+/+ controls fed either a high fat western-style diet (WD) or a zero fat, sucrose enriched diet (ZFD) for 24 weeks. (E–F) mRNA expression of genes involved in glycerol metabolism in inguinal (E) or epididymal (F) adipose tissue for male and female Mpc1^AD−/− mice or LoxP^+/+ controls fed a zero fat, sucrose enriched diet (ZFD). ∗∗P < 0.01, ∗∗∗P < 0.001 for Mpc1^AD−/− vs LoxP^+/+; ^#P < 0.05, ^###P < 0.001 main effect of genotype. Data are mean ± SE for 8-10 mice per group.