Figure 4.
31P relaxation characteristics of 30 mM NADH in binary mixed solvents of DMSO-d6 and D2O, with DMSO-d6 fractions ranging from 0.1 to 0.9, across temperatures from 277 to 343 K. (A) Spin–lattice relaxation rate (R1) as a function of temperature. The dashed line indicates the maximum R1 values observed at each solvent fraction. (B) Spin–spin relaxation rate (R2) as a function of temperature, showing an increase in R2 correlating with both higher fractions of DMSO-d6 and increased temperature.