Figure 5.

Comprehensive analysis of dynamic viscosity (η), diffusion coefficient (D), and hydrodynamic diameter (d_H). (A) Dynamic viscosity measurements of binary mixed solvents at 298 K. (B) ³¹P DOSY results for 30 mM NADH in the binary mixed solvents. (C) Hydrodynamic diameter extracted from the diffusion coefficients in (B) for 30 mM NADH in the binary mixed solvents, providing a quantitative measure of the molecular size of NADH in various solvent compositions. Arrows indicate specific points where ³¹P peak splitting from a singlet to a doublet of doublets is observed, specifically at 70%, 80%, and 90% DMSO-d₆ concentrations.