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. 2024 Sep 9;25:842. doi: 10.1186/s12864-024-10767-4

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Sequencing statistics of zooplankton metabarcoding with Illumina MiSeq and Nanopore MinION. (a) Bar plot of sequence reads demultiplexed per sample per sequencing dataset. (b) Species accumulation curves of molecular operational taxonomic unit (MOTU) richness for each sequencing platform against the number of samples, extrapolated to visualise number of samples needed to capture maximum richness; number of MOTUs obtained (and shared) expressed in Venn (insert). (c) Bar plots showing the number of MOTUs obtained per sample per sequencing type. (d) Box plots comparing MOTU richness, Simpson index, and Shannon-Weiner index between sequencing platforms; asterisks indicate significant differences for paired Wilcoxon signed-rank tests, and dots represent individual sample points (jittered)