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. 2024 Sep 9;57:64. doi: 10.1186/s40659-024-00544-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — TM and Testosterone Synthesis Genes. A: A schematic representation of the complete pathway for de novo testosterone synthesis; B: A UMAP plot showing the distribution of different cell clusters in the testis, with each colored dot representing a cell; C: The estimated proportions of various cell types following deconvolution analysis of transcriptomic sequencing from three groups of TM; D: A scatter plot illustrating the correlation analysis results after scoring each cell for two gene sets; E: The results from targeted steroid hormone metabolomics revealed the levels of various steroid hormones in macrophage culture medium and in control blank culture medium