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. 2024 Sep 9;57:64. doi: 10.1186/s40659-024-00544-8

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — Construction and Validation of Macrophage Depletion Mouse Model. A: Schematic diagram of the construction of the testicular macrophage depletion model in mice; B: Flow cytometry validation of the proportion of F4/80 positive cells in the total testis of macrophage-depleted mice compared to control mice; C, D, E, F: Immunohistochemical analysis showing changes in spermatogonial stem cells (Plzf), Interstitial cells (Cyp17a1), Sertoli cells (Sox9), and Testosterone after macrophage depletion, with bar graphs summarizing the results of the immunohistochemical analysis