Figure 7.

Altered metabolism of asparagine and tryptophan in GF-MCD-B mice after bacterial inoculation.

(a) Stool levels of asparagine in mice without bacterial inoculation (GF-MCD, n = 11) or inoculated with the bacterial consortium (GF-MCD-B, n = 10). Error bars show median with interquartile range. Significance was assessed by the Mann-Whitney U test. (b) Spearman’s correlation between stool levels of asparagine and liver fibrosis scores. (c) Hepatic levels of tryptophan, kynurenine, and tryptophan-to-kynurenine ratio in GF-MCD and GF-MCD-B mice. Error bars show median with interquartile range. Significance was assessed by the Mann-Whitney U test. (d) Spearman’s correlation between hepatic levels of tryptophan, kynurenine, and the tryptophan-to-kynurenine ratio and liver fibrosis scores. (e) Spearman’s correlation between hepatic levels of tryptophan, kynurenine, and the tryptophan-to-kynurenine ratio, and total OTU sequence reads in the liver.