Table 3. Results of (G+C)% + ρ(CG) screening on M.jannaschii.

Method	Annotated RNAs found	Annotated RNAs missed	Total no. of other hits	Other hits overlapping >50% putative protein gene	Putative protein genes with BLASTX hits to multiple species
(G+C)% alone	44	0	41	15	6
(G+C)% and ρ(CG)	43	1	28	6	1

Summary of results of scanning the M.jannaschii genome for ncRNAs using (G+C)% or (G+C)% combined with ρ(CG). Annotated RNAs include tRNAs, rRNAs and SRP RNAs of which only six tRNA genes and one rRNA gene were included in the training set. One notes adding ρ(CG) screening causes one RNA to be missed while reducing the number of putative hits overlapping a protein gene from 15 to 6, of which only one shows homology using BLASTX to exons from multiple other species. Further details are in the text.