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[Preprint]. 2024 Aug 26:2024.08.26.609768. [Version 1] doi: 10.1101/2024.08.26.609768

Figure 4.

Figure 4.

Mutations in the RGG domain of HNRNPA2B1 make it more cytoplasmic and a more potent regulator of neurite RNA localization. (A) Domain organization of HNRNPA2B1 rescue constructs. RNA recognition motifs are designated with RRM. (B) Immunoblot of wildtype and RGG mutant HNRNPA2B1 rescue transgenes. (C) Immunofluorescence of wildtype and RGG mutant HNRNPA2B1 rescue transgenes. (D) Quantification of the fraction of HNRNPA2B1 localized to the cytoplasm with wildtype and RGG mutant rescue transgenes. (E) Neurite enrichment levels of select motor protein RNAs in HNRNPA2B1 knockout, wildtype rescue, and RGG mutant rescue cells. (F) Neurite enrichment levels for HNRNPA2B1-insensitive and HNRNPA2B1-target RNAs (as defined in figure 1E) in HNRNPA2B1 knockout, wildtype rescue, and RGG mutant rescue cells. (G) RNA expression levels for select motor proteins in the soma and neurites of HNRNPA2B1 knockout, wildtype rescue, and RGG mutant rescue cells. Values are shown relative to expression levels in wildtype rescue cells. (H) Compartment-specific RNA expression levels for HNRNPA2B1-insensitive and HNRNPA2B1-target RNAs (as defined in figure 1E) in HNRNPA2B1 knockout, wildtype rescue, and RGG mutant rescue cells. P-values were calculated using a Wilcoxon ranksum test. NS (not significant) represents p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** represents p < 0.0001.