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. 2024 Jun 18;9(14):e179010. doi: 10.1172/jci.insight.179010

Figure 1. Number, fold change, and clonality of TCR clonotypes in HSV lesion site and arm biopsies before and after vaccination.

Figure 1

(A) Schematic of vaccine study timeline and procedures. (B) CD4+ and (C) CD8+ T cell densities of biopsies from control skin at the time of enrollment and from the site of a symptomatic lesion (N = 4) are shown in comparison to HSV lesion site and control skin biopsies over the course of a 3-dose vaccine trial in 9 vaccine recipients. Each dot represents the mean of 3 counted sections in a single participant. Median and interquartile range are shown in gray. (D) Representative micrographs (original magnification, ×10) of CD4+ (green) and CD8+ (red) T cell density by immunofluorescence (IF) in HSV lesion site biopsies at specified time points before and after vaccination. CD4+ and CD8+ T cell IF from (E) control skin and (F) lesion site during a symptomatic HSV-2 outbreak. All images are from P4. Scale bars: 100 mm. (G) Total and (H) number of TCRβ clonotypes detected at >4 copies are shown from the HSV lesion site and arm biopsies. (I) Clonality calculated from Shannon entropy of the TCR repertoire from each sample. Each dot represents a single participant. Median and interquartile range are shown in gray. *P < 0.05 by Wilcoxon’s signed-rank test.