Figure S4.
RH5-specific mAbs do not show Fc effector function in a phagocytosis- and complement-based assay, related to Figure 3
(A) Percentage of THP-1 monocyte cells with intracellular merozoites after opsonization of merozoites with various anti-RH5 mAbs. The anti-MSP1 mAb 42D6 (Patel et al.62) was used as a positive control.
(B) Growth inhibition of 3D7 parasites with anti-RH5 mAbs in the presence of serum that was either non-heat inactivated or heat inactivated to eliminate complement activity. MAD8–502 and MAD8–151 were tested at 50 μg/mL, while the other 4 mAbs were tested at 200 μg/mL.