Figure S2.

Proficient checkpoint in mrc1-DSE and mrc1-KAF mutants and similarities of mrc1ΔHBS and mcm2-2A mutants, related to Figure 3

(A) Ten-fold serial dilutions of cell suspensions were spotted on HU-containing medium to estimate checkpoint proficiency.

(B) Ten-fold serial dilutions of cell suspensions were spotted on rich YES medium and incubated at the indicated temperatures to check for bypass of the hsk1-89 mutation at the restrictive temperature of 30°C.

(C) Asymmetric loss of heterochromatic gene silencing of (EcoRV)::mCherry reporter in mcm2-2A mutant visualized by time-lapse microscopy. Scale bar: 10 μm.

(D) Similar temperature-dependency of heterochromatic silencing in mrc1ΔHBS and mcm2-2A mutants.

In both (C) and (D), microcolonies were grown from single cells under a fluorescence microscope.