Figure S3.

Heterochromatin loss away from nucleation sites in Mrc1 and Mcm2 mutants, related to Figure 3

(A) H3K9me2 occupancy at Chromosome 1 (top) and Chromosome 2 (bottom) in histone-recycling mutants. siRNAs are depicted as red blocks across Tel1L/Tel2L (left), centromeres (middle), or Tel1R/Tel2R (right).

(B) H3K9me2 occupancy at the mating-type region indicating siRNAs originating from cenH depicted in red and the two Atf1-binding sites in magenta.

(C) Ten-fold serial dilutions of cell suspensions were spotted onto the indicated media. Growth on the toxigenic substrate FOA reflects ura4⁺ repression.

(D) H3K9me2 occupancy at the h⁹⁰ and ΔK mating-type regions.

(E) Quantification of H3K9me2 occupancy at indicated regions within the mating-type region normalized to cnt3 [unpaired Student’s t test used].

(F) Schematic diagram of Pob3 with functional domains annotated and deletion mutations indicated.

(G) Proportion of cells expressing mCherry in cells co-expressing Pob3-GBP (full-length and mutants) and Mrc1ΔHBS-GFP (mean ± SD, n = 6) [ANOVA, F = 41.6, P = 8.7 × 10⁻⁹].